Optimized production of 2,3-butanediol by a lactate dehydrogenase-deficient mutant of Klebsiella pneumoniae

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논문

Optimized production of 2,3-butanediol by a lactate dehydrogenase-deficient mutant of Klebsiella pneumoniae

학술지

Biotechnology and bioprocess engineering

저자명

Lee, Sung-Mok; Oh, Baek-Rock; Park, Jang Min; Yu, Anna; Heo, Sun-Yeon; Hong, Won-Kyung; Seo, Jeong-Woo; Kim, Chul Ho

초록

To obtain high-yield production of 2,3-butanediol (2,3-BD) from glucose, we optimized the culture conditions for a lactate dehydrogenase-deficient mutant (<TEX>${\Delta}ldhA$</TEX>) of Klebsiella pneumoniae using response surface methodology. 2,3-BD production was successfully improved by optimizing pH (5.6), aeration (3.50 vvm) and concentration of corn steep liquor (45.0 mL/L) as a nitrogen source, resulting in a maximum level of 2,3-BD production of 148.8 g/L and productivity of 2.48 g/L/h. 2,3-BD was also obtained with high concentration (76.24 g/L) and productivity (2.31 g/L/h) from the K. pneumoniae mutant strain using sugarcane molasses as a carbon source.

발행연도

2013

발행기관

Korean Society for Biotechnology and Bioengineering

ISSN

1226-8372

권

호

페이지

pp.1210-1215

DOI

10.1007/s12257-013-0396-z