초록
<P><B>Abstract</B></P> <P>In this study, the expression of 4 genes encoding α-acetolactate synthase, α-acetolactate decarboxylase, 2,3-butanediol dehydrogenase, and NADH oxidase was modulated using a previously developed cocktail δ-integration strategy. The resultant strain, YPH499/dPdAdG/BD6-10, was used in a fed-batch cultivation for the production of 2,3-butanediol. The concentration, production rate, and yield obtained were 80.0g/L, 4.00g/L/h, and 41.7%, respectively. The production rate and yield of the compound obtained are higher for this strain compared to reports published for <I>Saccharomyces cerevisiae</I> so far. The cocktail δ-integration strategy allows for modulation of multiple gene expression, without the exact knowledge of rate-limiting steps, and therefore, could be used as a promising strategy for the production of bio-based chemicals in recombinant <I>S. cerevisiae</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 2,3-Butanediol (BDO) is useful metabolites produced by microorganisms. </LI> <LI> BDO production by <I>Saccharomyces cerevisiae</I> was examined. </LI> <LI> Expression of 4 genes was optimized by cocktail δ-integration strategy. </LI> <LI> BDO productivity was improved by cocktail δ-integration strategy. </LI> <LI> The highest BDO production rate and yield were achieved in fed-batch cultivation. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>