Methane production from lipid extracted, pre-treated disrupted and non-pretreated Tetraselmis spp. microalgae was investigated. The results demonstrated that 122mLpergVS methane was produced for the lipid extracted Tetraselmis spp., demonstrating that lipid free Tetraselmis can be effectively digested in an anaerobic environment. A total of 252mLpergVS and 248mLpergVS of methane was reported for non-disrupted and pre-treated disrupted Tetraselmis sp. respectively. It was also observed that the microbial community caused cell lysis of Tetraselmis spp. during the anaerobic digestion process. Cell lyses can offer a direct conversion pathway of intact Tetraselmis spp. for energy production, thus negating the need for pre-treatment.