Microbial community analysis of a methane-producing biocathode in a bioelectrochemical system
메타 데이터
바이오화학분류
바이오정밀화학
연료
논문
Microbial community analysis of a methane-producing biocathode in a bioelectrochemical system
학술지
Archaea : an international microbiological journal
저자명
Van Eerten-Jansen, Mieke C. A. A.; Veldhoen, Anna B.; Plugge, Caroline M.; Stams, Alfons J. M.; Buisman, Cees J. N.; Ter Heijne, Annemiek
초록
<P>A methane-producing biocathode that converts CO<SUB>2</SUB> into methane was studied electrochemically and microbiologically. The biocathode produced methane at a maximum rate of 5.1 L CH<SUB>4</SUB>/m<SUP>2</SUP> projected cathode per day (1.6 A/m<SUP>2</SUP>) at −0.7 V versus NHE cathode potential and 3.0 L CH<SUB>4</SUB>/m<SUP>2</SUP> projected cathode per day (0.9 A/m<SUP>2</SUP>) at −0.6 V versus NHE cathode potential. The microbial community at the biocathode was dominated by three phylotypes of Archaea and six phylotypes of bacteria. The Archaeal phylotypes were most closely related to <I>Methanobacterium palustre</I> and <I>Methanobacterium aarhusense</I>. Besides methanogenic Archaea, bacteria seemed to be associated with methane production, producing hydrogen as an intermediate. Biomass density varied greatly with part of the carbon electrode covered with a dense biofilm, while only clusters of cells were found on other parts. Based on our results, we discuss how inoculum enrichment and changing operational conditions may help to increase biomass density and to select for microorganisms that produce methane.</P>