Cre-lox66/lox71-Based Elimination of Phosphotransacetylase or Acetaldehyde Dehydrogenase Shifted Carbon Flux in Acetogen Rendering Selective Overproduction of Ethanol or Acetate

BioChem - DOS

Search

메타 데이터

바이오화학분류

- 바이오플라스틱
  1. 플라스틱
- 바이오정밀화학
  1. 용매
  2. 화학제품
  3. 연료
- 화장품용 기능성소재
  1. 계면활성제⁄증점제
- 의료용 화학소재
  1. 식품첨가제

논문

Cre-lox66/lox71-Based Elimination of Phosphotransacetylase or Acetaldehyde Dehydrogenase Shifted Carbon Flux in Acetogen Rendering Selective Overproduction of Ethanol or Acetate

학술지

Applied biochemistry and biotechnology

저자명

Berzin, Vel; Kiriukhin, Michael; Tyurin, Michael

초록

<P>Acetogen strain Clostridium sp. MT1121 produced 300 mM acetate (p<0.005) and 321 mM ethanol (p<0.005) from synthesis gas (syngas) blend 60 % CO and 40 % H(2). Clostridium sp. MT1121 was metabolically engineered to eliminate production of either acetate or acetaldehyde during syngas fermentation. We used Cre-lox66/lox71-based gene removal system to eliminate either phosphotransacetylase (pta), or acetaldehyde dehydrogenase (aldh). The resulted biocatalyst with eliminated pta increased ethanol yield to 610 mM (p<0.005). Inactivation of pta rendered only 502 mM of ethanol (p<0.005). The acetogen biocatalyst with eliminated aldh produced 450 mM acetate (p<0.005). The role of cell energy pool preservation for re-directed carbon flux is discussed. This is the first report on time- and cost-efficient gene elimination in acetogens using lox66/lox71 gene elimination system.</P>

발행연도

2012

발행기관

Springer-Verlag

ISSN

0273-2289

ISSN

1559-0291

권

168

호

페이지