초록
<P>Trichoderma reesei YC-108, a strain isolated by a kind of newly invented plate was found to over produce cellulase and it was then used as a cellulase producer. To get the maximum amount of cellulase, the combination of the medium ingredients, which has a profound influence on metabolic pathway was optimized using response surface methodology. The optimum composition was found to be 24.63?g/L wheat bran, 30.78?g/L avicel, and 19.16?g/L soya-bean cake powder. By using the optimized medium, the filter paper activity (FPA) increased nearly five times to 15.82?IU/mL in a 30?L stirred fermenter, carboxymethyl cellulase activity (CMCase) was increased from 83.02 to 628.05?IU/mL and the CMCase/FPA ratio was nearly doubled compared with the parent strain at initial medium.</P>