초록
<P><B>Abstract</B></P> <P>Butanol production from glycerol was investigated through <I>Clostridium pasteurianum</I> entrapped into polyvinyl alcohol particles. Using an optimized system, batch and repeated batch fermentations with free and entrapped cells were performed, respectively. In both systems, glycerol samples of different purity were tested. In repeated batch fermentations, process time decreased from 19.5 to 2.7 h and butanol productivity increased 6.3 times (3.08 g L<SUP>−1</SUP> h<SUP>−1</SUP>) compared with the free-cell process, using pure glycerol. In the case of glycerol from biodiesel production, butanol productivity of 2.90 and 1.76 g L<SUP>−1</SUP> h<SUP>−1</SUP> was achieved from glycerol 01 and glycerol 02, respectively. No cell growth or solvent production was observed when the same glycerols were used in free-cell fermentations.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Process development of <I>Clostridium pasteurianum</I> entrapment to polyvinyl alcohol particles. </LI> <LI> Entrapped cells reached 6.3 times higher butanol productivity from glycerol than free cells. </LI> <LI> Entrapped cells successfully applied to crude glycerol. </LI> <LI> Immobilization matrix preserves the biomass from the toxic effect of the substrate. </LI> </UL> </P>