초록
<P><B>Abstract</B></P> <P> <I>Saccharomyces cerevisiae</I>, a recombinant <I>S. cerevisiae</I>, <I>Pichia stipistis</I> and an adapted <I>P. stipistis</I> were compared in fermenting steam exploded corn stover (SECS) enzymatic hydrolysate, which contained glucose and xylose, among which <I>S. cerevisiae</I> was found to be the most efficient. The residual xylose in the fermentation broth of <I>S. cerevisiae</I> was concentrated during the distillation of ethanol and fermented by xylose-fermenting yeasts, among which the adapted <I>P. stipistis</I> was found to be the best. As a result, a novel and efficient bioprocess from SECS to ethanol was established, producing approximately 27.00 g ethanol from 111.51 g SECS with an ethanol production rate of 2.50 g/L/h and a yield of 0.24 g ethanol/g SECS, and 58.18 g ethanol from 341.45 g SECS with an ethanol production rate of 2.31 g/L/h and a yield of 0.17 g ethanol/g SECS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel and efficient bioprocess from SECS to ethanol was successfully established. </LI> <LI> On-site cellulase production was done by the mixed culture of <I>T. reesei</I> and <I>A. niger.</I> </LI> <LI> <I>S. cerevisiae</I> was the most robust in the fermentation of SECS enzymatic hydrolysate. </LI> <LI> The adapted <I>P. stipistis</I> was the most robust in fermenting the distillation residue. </LI> <LI> Xylose-fermenting <I>S. cerevisiae</I> was constructed and <I>P. stipistis</I> was adapted. </LI> </UL> </P>