초록
Three continuous stirred tank reactors (CSTR) were operated under mesophilic (37+/-1<SUP>o</SUP>C), thermophilic (55+/-1<SUP>o</SUP>C) and hyper-thermophilic (80+/-1<SUP>o</SUP>C) temperatures for 164days to investigate the effect of temperature and temperature shock on the cellulosic-dark hydrogen fermentation by mixed microflora. During steady state condition, the sudden decreases in the fermentation temperature occurred twice in each condition for 24h. The results show that the 55+/-1 and 80+/-1<SUP>o</SUP>C presented stable hydrogen yields of 12.28 and 9.72mmol/g cellulose, respectively. However, the 37+/-1<SUP>o</SUP>C presented low hydrogen yield of 3.56mmol/g cellulose and methane yield of 5.4mmol/g cellulose. The reactor performance under 55+/-1 or 80+/-1<SUP>o</SUP>C appeared to be more resilient to the sudden decreases in the fermentation temperature than 37+/-1<SUP>o</SUP>C. The experimental analysis results indicated that the changing in soluble by-products could explain the effect of temperature and temperature shock, and the thermophilic temperature is expected having a better economic performance for cellulosic-hydrogen fermentation.