Self-assembly of bio-cellulose nanofibrils through intermediate phase in a cell-free enzyme system
메타 데이터
바이오화학분류
바이오플라스틱
기타
바이오정밀화학
기타
화장품용 기능성소재
기타
의료용 화학소재
식품첨가제
논문
Self-assembly of bio-cellulose nanofibrils through intermediate phase in a cell-free enzyme system
학술지
Biochemical engineering journal
저자명
Kim, Yeji; Ullah, Muhammad Wajid; Ul-Islam, Mazhar; Khan, Shaukat; Jang, Jae Hyun; Park, Joong Kon
초록
<P><B>Abstract</B></P> <P>The current study was aimed to investigate the synthesis and self-assembly of cellulose nanofibrils in <I>in vitro</I> environment through intermediate phase analysis and compare with the microbial cell system. The presence and stability of cellulose synthase and other essential enzymes were qualitatively validated through liquid chromatography–mass spectrometry/mass spectrometry linear trap quadrupole (LC–MS/MS LTQ) Orbitrap analysis of the culture medium after each batch (15 days’ point) of <I>in vitro</I> incubation. Monitoring of bio-cellulose synthesis in the <I>in vitro</I> environment revealed that β-1,4-glucan chains were <I>de novo</I> synthesized within the culture medium which crystallized and formed the premature bio-cellulose and bio-cellulose pellicles. The pellicle moved to the surface of the medium and ultimately formed a bio-cellulose sheet at the air-medium interface. In contrast to aerobic synthesis of cellulose (bacterial cellulose) by microbial cells, bio-cellulose was synthesized anaerboically by the cell-free enzyme system and the thickness of the sheet at the air-medium interface increased from the bottom surface facing the culture medium as confirmed by the field-emission scanning electron microscopy (FE-SEM). The current study will provide a base for <I>in situ</I> development of bionanocomposites of cellulose with various bactericidal and polymeric materials for broad spectrum applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Submerged production and self-aggregation of bio-cellulose nanofibrils. </LI> <LI> <I>In vitro</I> analysis of cellulose nanofibrils aggregation through intermediate phase. </LI> <LI> Stability of bio-cellulose synthesis enzyme in repeated batch operations. </LI> <LI> Anaerobic synthesis of bio-cellulose and comparison with microbial cell system. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>