초록
<P><B>Abstract</B></P> <P>Medium-chain alcohols are used to produce solvents, surfactants, lubricants, waxes, creams, and cosmetics. In this study, we engineered the oleaginous yeast <I>Yarrowia lipolytica</I> to produce 1-decanol from glucose. Expression of a fatty acyl-CoA reductase from <I>Arabidopsis thaliana</I> in strains of <I>Y. lipolytica</I> previously engineered to produce medium-chain fatty acids resulted in the production of 1-decanol. However, the resulting titers were very low (<10mg/mL), most likely due to product catabolism. In addition, these strains produced small quantities of 1-hexadecanol and 1-octadecanol. Deleting the major peroxisome assembly factor Pex10 was found to significantly increase 1-decanol production, resulting in titers exceeding 500mg/L. It also increased 1-hexadecanoland and 1-octadecanol titers, though the resulting increases were less than those for 1-decanol. These results demonstrate that <I>Y. lipolytica</I> can potentially be used for the industrial production of 1-decanol and other fatty alcohols from simple sugars.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The oleaginous yeast <I>Yarrowia lipolytica</I> was engineered to produce medium and long-chain fatty alcohols. </LI> <LI> 1-decanol, 1-hexadecanol, and 1-octodecanol were produced. </LI> <LI> 1-decanol titers were increased nearly 100-fold by eliminating product catabolism. </LI> <LI> The maximum titer for 1-decanol was 550mg/L. </LI> </UL> </P>