Metabolic engineering of Klebsiella oxytoca M5a1 to produce optically pure D-lactate in mineral salts medium

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논문

Metabolic engineering of Klebsiella oxytoca M5a1 to produce optically pure D-lactate in mineral salts medium

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Sangproo, M.; Polyiam, P.; Jantama, S.S.; Kanchanatawee, S.; Jantama, K.

초록

Klebsiella oxytoca strains were constructed to produce optical pure d-lactate by pH-controlled batch fermentation in mineral salts medium. The alcohol dehydrogenase gene, adhE, and the phospho-transacetylase/acetate kinase A genes, pta-ackA, were deleted from the wild type. KMS002 (ΔadhE) and KMS004 (ΔadhE Δpta-ackA) exhibited d-lactate production as a primary pathway for the regeneration of NAD<SUP>+</SUP>. Both strains produced 11-13g/L of d-lactate in medium containing 2% (w/v) glucose with yields of 0.64-0.71g/g glucose used. In sugarcane molasses, KMS002 and KMS004 produced 22-24g/L of d-lactate with yields of 0.80-0.87g/g total sugars utilized. Both strains also utilized maltodextrin derived from cassava starch and produced d-lactate at a concentration of 33-34g/L with yields of 0.91-0.92g/g maltodextrin utilized. These d-lactate yields are higher than those reported for engineered E. coli strains.

발행연도

2012

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

119

페이지

pp.191-198

DOI

10.1016/j.biortech.2012.05.114