초록
<P><B>Abstract</B></P> <P>5-hydroxymethylfurfural (5-HMF), a key precursor to biochemicals has been produced from simple sugars. We report the production of 5-HMF directly from sustainable <I>Chlorella sorokiniana</I> cultivated in a saline medium supplied with CO<SUB>2</SUB> as the sole carbon source. 43% carbohydrate and 16% lipid production in <I>C. sorokiniana</I> were achieved with an optimized CO<SUB>2</SUB> concentration of 1%. 5-HMF yield of 52% was produced in a one-pot acid dehydration reactor at 150 °C in 2 h. 5-HMF yield from <I>C. sorokiniana</I> was almost a 40-fold higher than that from lignocellulose sugar cane bagasse – a highly abundant biomass. SEM images revealed that, the cells of <I>C. sorokiniana</I> could easily be broken in this one-pot reactor to liberate the built-up carbohydrate from the plastids of the cells for subsequent conversion to 5-HMF. The presence of lipids in <I>C. sorokiniana</I> was found to promote the conversion of starch to 5-HMF. Ultimately, the addition of LiCl was found to produce more than 2-fold 5-HMF from <I>C. sorokiniana</I> when compared to the absence of metal salts, with a 5-HMF selectivity of 98.7%. These findings suggest that, starch accumulating microalgae could be potential sustainable feedstock for 5-HMF production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 1% CO<SUB>2</SUB> feed resulted in 43.3% carbohydrate content in <I>Chlorella sorokiniana</I>. </LI> <LI> 5-HMF was produced from <I>C. sorokiniana</I> in a pseudo biphasic system. </LI> <LI> The presence of lipids enhances the formation of 5-HMF. </LI> <LI> 52.4% 5-HMF yield was obtained with 200 mg/mL LiCl. </LI> <LI> 5-HMF selectivity reached 98.7% with saturated LiCl. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>