BioChem - DOS

Enzymatic N-Glycosylation of Diverse Arylamine Aglycones by a Promiscuous Glycosyltransferase from Carthamus tinctorius

Advanced synthesis & catalysis

Xie, Kebo; Chen, Ridao; Chen, Dawei; Li, Jianhua; Wang, Ruishan; Yang, Lin; Dai, Jungui

<P><B>Abstract</B></P><P>A new glycosyltransferase (UGT71E5) from <I>Carthamus tinctorius</I> exhibited a robust promiscuity towards 30 structurally diverse drug&#8208;like aromatic amine scaffolds, making it the first reported glycosyltransferase capable of catalyzing <I>N</I>&#8208;glycosylation with multiple diverse nitrogen&#8208;heterocyclic aromatic compounds. The catalytic promiscuity and reversibility of UGT71E5 was exploited to generate the rare <I>N</I>&#8208;glycoside from the abundant <I>O</I>&#8208;glycoside with high efficiency. These findings demonstrate the significant potential of UGT71E5 in the enzymatic synthesis of diverse bioactive <I>N</I>&#8208;glycosides.</P>

2017

1615-4150

1615-4169

359

4

pp.603-608

10.1002/adsc.201601128

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77401413

Carthamus tinctorius; catalytic promiscuity; enzyme catalysis; glycosylation; glycosyltransferases; N-glycosylation