BioChem - DOS

Saccharides in straw hydrolysate decrease cell membrane damage by phenolics by inducing the formation of extracellular matrix in yeast

Carbohydrate polymers

Wang, Xiahui; Cui, Shouqing; Hu, Jiajun; Ma, Xiubing; Zhang, Tian-Ao; Tsang, Yiu Fai; Li, Jixiang; Gao, Min-Tian

<P><B>Abstract</B></P> <P>The bioconversion of rice straw into ethanol can alleviate the energy crisis and solve problems related to waste treatment. In this study, the effect of soluble polysaccharides (SPs) produced during rice straw saccharification on the formation of extracellular matrices (EMs) by the yeast <I>Saccharomyces cerevisiae</I> was investigated. SPs were characterized by high-performance liquid chromatography (HPLC) and fourier transform infrared spectroscopy (FT-IR). SPs reduced the inhibition of alcohol dehydrogenase activity by phenolic acids (PAs) and regulated the intracellular redox state, resulting in higher ethanol production. The results of flow cytometry, confocal laser scanning microscopy, and atomic force microscopy indicated that PAs changed microbial morphology and caused damage in microbial cell membranes. The protective effect of SPs against cell membrane damage could be attributed to the synthesis of polysaccharide-dependent extracellular matrix, which maintained cellular integrity even under phenolic acid stress. These findings provide new strategies to improve pretreatment and saccharification processes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Soluble polysaccharides (SPs) were obtained from rice straw. </LI> <LI> Xylan and glucan were the main components of SPs. </LI> <LI> SPs enhanced the formation of polysaccharide-dependent extracellular matrix. </LI> <LI> Extracellular matrix secreted by <I>Saccharomyces cerevisiae</I> maintained cellular integrity even under phenolic acid stress. </LI> <LI> SPs increased ethanol production by <I>S. cerevisiae</I>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>SPs could alleviate inhibition of phenolic acids, leading to high fermentability.</P> <P>[DISPLAY OMISSION]</P>

2019

Elsevier

0144-8617

219

pp.414-422

10.1016/j.carbpol.2019.05.030

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART106547006

Ethanol fermentation; Phenolic acids; Polysaccharides; Protective effects; Rice straw