BioChem - DOS

Optimization of culture conditions for Aspergillus sojae expressing an Aspergillus fumigatus α-galactosidase

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Gurkok, S.; Cekmecelioglu, D.; Ogel, Z.B.

Using Response Surface Methodology, carbon and nitrogen sources and agitation speed for cultivation of Aspergillus sojae expressing the &alpha;-galactosidase gene, aglB of Aspergillus fumigatus IMI 385708 were optimized. Compared to cultivation in modified YpSs medium, cultivation in 250-mL Erlenmeyer flasks agitated at 276rpm and containing 100mL of optimized medium consisting of 10.5% molasses (w/v) and 1.3% NH<SUB>4</SUB>NO<SUB>3</SUB> (w/v), 0.1% K<SUB>2</SUB>HPO<SUB>4</SUB>, and 0.005% MgSO<SUB>4</SUB>.7H<SUB>2</SUB>O achieved a 4-fold increase in &alpha;-galactosidase production (10.4U/mL). These results suggest the feasibility of industrial large scale production of an &alpha;-galactosidase known to be valuable in galactomannan modification.

2011

Elsevier Applied Science

0960-8524

102

7

pp.4925-4929

10.1016/j.biortech.2011.01.036

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART55883658

α-Galactosidase; Aspergillus; Optimization; Response surface methodology (RSM); Box-behnken design (BBD)