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Effects of pH and fermentation strategies on 2,3-butanediol production with an isolated Klebsiella sp. Zmd30 strain

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논문

Effects of pH and fermentation strategies on 2,3-butanediol production with an isolated Klebsiella sp. Zmd30 strain

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Wong, C.L.; Yen, H.W.; Lin, C.L.; Chang, J.S.

초록

This study examined the effects of pH-controlled and fermentation strategies on 2,3-butanediol (2,3-BDO) production by an isolated Klebsiella sp. Zmd30 strain. The pH value of 6.0 was found to be optimal among the investigated range of 4.5-9.0. In batch fermentation, the concentration, productivity and yield of 2,3-BDO were 57.17g/l, 1.59g/l/h and 82%, respectively. The 2,3-BDO production by Klebsiella sp. Zmd30 was found to be growth-associated. Higher 2,3-BDO concentration (110g/l) and yield (94%) was obtained by using fed-batch operation, but the productivity was lower (0.88g/l/h) as compared to that when using batch operation. The highest 2,3-BDO productivity of 2.81g/l/h was obtained with the continuous culture at a hydraulic retention time (HRT) of 12h. The results suggest that fed-batch operation might be most suitable for commercialized 2,3-BDO production, due to obtaining a high concentration and yield.

발행연도

2014

발행기관

Elsevier Applied Science

ISSN

0960-8524

152

페이지

pp.169-176

주제어

2,3-Butanediol; Klebsiella sp.; Fermentation mode; pH control

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논문; 2014-01-01

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