초록
<P>Xylooligosaccharides as emerging prebiotics are able to promote the growth of probiotic bacteria. In the present study, four neutral, thermostable xylanases (<I>Mt</I>Xyn11A, <I>Mt</I>Xyn11At, <I>Mt</I>Xyn11B, and <I>Mt</I>Xyn11C) from compost fungus <I>Mycothermus thermophilus</I> CGMCC3.18119 were overexpressed in <I>Pichia pastoris</I> GS115 and used to produce xylooligosaccharides from beechwood xylan. The enzymes showed similar enzymatic properties (maximal activities at pH 6.0–6.5 and 65 °C) but varied in catalytic efficiency and cleaving actions. <I>Mt</I>Xyn11A, <I>Mt</I>Xyn11At, and <I>Mt</I>Xyn11C mainly produced xylobiose (59–62%), xylose (16–20%), and xylotriose (16–19%), while <I>Mt</I>Xyn11B released xylobiose (51%), xylotriose (32%), and xylose (12%) as the main products. When using the xylan hydrolysates of different xylanases as the carbon source, four probiotic <I>Lactobacillus</I> strains <I>Lactobacillus brevis</I> 1.2028, <I>Lactobacillus rhamnosus</I> GG, <I>Lactobacillus casei</I> BL23, and <I>Lactobacillus plantarum</I> WCSF1 were confirmed to use the xylooligosaccharides efficiently (83.8–98.2%), with <I>L. brevis</I> 1.2028 as the greatest.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2017/jafcau.2017.65.issue-6/acs.jafc.6b05183/production/images/medium/jf-2016-05183w_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf6b05183'>ACS Electronic Supporting Info</A></P>