초록
<P><B>Abstract</B></P> <P>In the present study, a <I>Clostridium beijerinckii</I> strain capable of producing Isopropanol-Butanol (IB) was isolated from gram grain. Batch fermentation of un-detoxified sulfuric acid pretreated rice straw (RS) hydrolysate (SARSH) with this strain resulted in the production of 6.6g L<SUP>−1</SUP> acetone-free Isopropanol-Butanol (IB), of which butanol was 4.9 g L<SUP>−1</SUP>. Fermentation of hydrolysate obtained after ammonia pretreated enzyme hydrolysed RS resulted in the production of 8.5 g L<SUP>−1</SUP> of acetone-free IB, giving a yield of 0.34 g g<SUP>−1</SUP>. IB production from fermentation of 30 g L<SUP>−1</SUP> glucose and 30 g L<SUP>−1</SUP> xylose was 10 and 8.6 g L<SUP>−1</SUP>, respectively, showing an equal and efficient utilization of both glucose and xylose. The total solvent yield from glucose and xylose was 0.36 and 0.32 g g<SUP>−1</SUP> respectively. During fermentation mostly butanol (7.0 g L<SUP>−1</SUP>) and isopropanol (2.0 g L<SUP>−1</SUP>) were produced as biosolvents with negligible amounts of ethanol (0.1 g L<SUP>−1</SUP>). Since isopropanol can be used as a fuel additive, the mixture of isopropanol and butanol (IB) can be directly used as a biofuel.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Isolation of a wild-type <I>Clostridium beijerinckii</I> strain C-01. </LI> <LI> Strain C-01 distinguishes itself by being a non-acetone forming isopropanol-butanol producer. </LI> <LI> Strain C-01 also distinguishes itself by being able to efficiently utilize xylose as well as glucose simultaneously. </LI> <LI> Efficient IB produced from Rice straw hydrolysate derived medium. </LI> </UL> </P>