초록
<P><B>Abstract</B></P> <P>In this study, bacterium <I>Pseudomonas</I> sp. strain D was proved to be the main partner assisting <I>Chlamydomonas reinhardtii</I> in improving photolysis-mediated H<SUB>2</SUB> production and a good partner for promoting H<SUB>2</SUB> production by the green algae <I>Chlorella</I> and <I>Scenedesmus</I>. In strain D partnered algal-bacterial co-culture, the relative O<SUB>2</SUB> content in the headspace plus the dissolved oxygen in the culture medium were rapidly consumed by bacterial growth, resulting in a completely anaerobic environment that proved suitable for the activation of algal hydrogenase. Moreover, algal-bacterial cooperation was able to slow the reduction of chlorophyll, enhance starch accumulation, and maintain protein content, which are the potential factors whose control provides an opportunity for improving algal H<SUB>2</SUB> production. This study systematically analyzed the main pathway responsible for H<SUB>2</SUB> production by algal-bacterial cooperation and the potential mechanisms for improvement, and proposed an efficient and durable algal-bacterial cooperation system for improving photolysis-mediated H<SUB>2</SUB> production by green algae.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Algal-bacterial cooperation improves the photolysis H<SUB>2</SUB> production. </LI> <LI> Completely anaerobic culture condition was built by bacterial partner. </LI> <LI> Algal-bacterial cooperation slows chlorophyll reduction and maintains protein. </LI> <LI> Direct water photolysis contributes mainly to the algal H<SUB>2</SUB> production. </LI> </UL> </P>