BioChem - DOS

Temporal dynamics and metabolic correlation between lactate-producing and hydrogen-producing bacteria in sugarcane vinasse dark fermentation: The key role of lactate

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Fuess, Lucas Tadeu; Ferraz Jú nior, Antô nio Djalma Nunes; Machado Jú nior, Carla Botelho; Zaiat Jú nior, Marcelo

<P><B>Abstract</B></P> <P>This study aimed to better understand the role of different microbial groups and the determining fermentation pathways in a biohydrogen (bioH<SUB>2</SUB>)-producing reactor fed with sugarcane vinasse by using next-generation sequencing and principal component analysis (PCA). Both microbial structure and dynamics were characterized. The highest bioH<SUB>2</SUB> production levels were associated with the <I>Thermoanaerobacterium</I> genus, whilst lactate-producing bacteria comprised the dominant genera (e.g. <I>Lactobacillus</I> and <I>Leuconostoc</I>) within the reactor at both stable and unstable bioH<SUB>2</SUB>-producing periods. PCA further revealed that the fermentation of lactate played a dual role in the reactor, as both bioH<SUB>2</SUB>-producing (acetate+lactate&rarr;butyrate+bioH<SUB>2</SUB>) and non-bioH<SUB>2</SUB>-producing (lactate&rarr;propionate+acetate) routes could be observed. Overall, the results suggested that lactate is the primary alternative carbon source in vinasse-fed systems subjected to carbohydrate-shortage conditions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The microbial structure and dynamics of vinasse dark fermentation was characterized. </LI> <LI> Lactate was the primary alternative carbon source for acidogenic bacteria. </LI> <LI> H<SUB>2</SUB>-producing and non-H<SUB>2</SUB>-producing pathways from lactate were identified. </LI> <LI> The highest H<SUB>2</SUB> production levels were related with the <I>Thermoanaerobacterium</I> genus. </LI> <LI> Biomass discharges did not favor the growth of the <I>Thermoanaerobacterium</I> genus. </LI> </UL> </P>

2018

Elsevier

0960-8524

247

pp.426-433

10.1016/j.biortech.2017.09.121

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART79612485

Sugarcane vinasse; Biohydrogen production; Lactate-producing bacteria; Next-generation sequencing; MiSeq Illumina