BioChem - DOS

Metabolic engineering of fatty acyl-ACP reductase-dependent pathway to improve fatty alcohol production in Escherichia coli

Metabolic engineering

Liu, R.; Zhu, F.; Lu, L.; Fu, A.; Lu, J.; Deng, Z.; Liu, T.

Fatty alcohols are important components of surfactants and cosmetic products. The production of fatty alcohols from sustainable resources using microbial fermentation could reduce dependence on fossil fuels and greenhouse gas emission. However, the industrialization of this process has been hampered by the current low yield and productivity of this synthetic pathway. As a result of metabolic engineering strategies, an Escherichia coli mutant containing Synechococcus elongatus fatty acyl-ACP reductase showed improved yield and productivity. Proteomics analysis and in vitro enzymatic assays showed that endogenous E. coli AdhP is a major contributor to the reduction of fatty aldehydes to fatty alcohols. Both in vitro and in vivo results clearly demonstrated that the activity and expression level of fatty acyl-CoA/ACP reductase is the rate-limiting step in the current protocol. In 2.5-L fed-batch fermentation with glycerol as the only carbon source, the most productive E. coli mutant produced 0.75g/L fatty alcohols (0.02g fatty alcohol/g glycerol) with a productivity of up to 0.06g/L/h. This investigation establishes a promising synthetic pathway for industrial microbial production of fatty alcohols.

2014

Academic Press

1096-7176

1096-7184

22

pp.10-21

10.1016/j.ymben.2013.12.004

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART68796060

Fatty acyl-CoA/ACP reductase; In vitro reconstitution; Fatty aldehyde reductase; AdhP; Fatty alcohol