BioChem - DOS

SK-HEP cells and lentiviral vector for production of human recombinant factor VIII

Biotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology

da Rosa, Nathalia Gonsales; Swiech, Kamilla; Picanç o-Castro, Virgí nia; de Sousa Russo-Carbolante, Elisa Maria; Neto, Mario Abreu Soares; de Castilho-Fernandes, Andrielle; Faç a, Vitor Marcel; Fontes, Aparecida Maria; Covas, Dimas Tadeu

<P>Hemophilia A is caused by a deficiency in coagulation factor VIII. Recombinant factor VIII can be used as an alternative although it is unavailable for most patients. Here, we describe the production of a human recombinant B-domain-deleted FVIII (rBDDFVIII) by the human cell line SK-HEP-1, modified by a lentiviral vector rBDDFVIII was produced by recombinant SK-HEP cells (rSK-HEP) at 1.5-2.1 IU/10(6) in 24 h. The recombinant factor had increased in vitro stability when compared to commercial pdFVIII. The functionality of rBDDFVIII was shown by its biological activity and by tail-clip challenge in hemophilia A mice. The rSK-HEP cells grew in a scalable system and produced active rBDDFVIII, indicating that this platform production can be optimized to meet the commercial production scale needs.</P>

2012

Springer-Verlag

0141-5492

1573-6776

34

8

pp.1435-1443

10.1007/s10529-012-0925-4

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