초록
<P><B>Abstract</B></P> <P>As a perennial grass, <I>Humulus scandens</I> is rich in cellulose which can be fermented for bioenergy producing. Hence, the hydrogen production potential of the <I>Humulus scandens</I> from dark fermentation by <I>Enterobacter aerogenes</I> was investigated in this paper. Cellulase amount, inoculation amount, and initial pH value were evaluated. The interrelationship between these factors were studied by Response Surface Box-Behnken Experiments. Results showed that there was a significant correlation between the three factors. Through the correction of the regression equation, the optimized technological conditions were obtained. The amount of cellulase was 0.203 g g<SUP>−1</SUP> TS, the inoculation amount was 42.6%, the initial pH value was 6.59, the pre-estimated maximum cumulative value of hydrogen production was 65.12 mL g<SUP>−1</SUP> TS, and it was similar to the test mean value which was 64.08 mL g<SUP>−1</SUP> TS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Humulus scandens</I> can be used for biohydrogen production. </LI> <LI> Three factors affecting bio-hydrogen production of <I>Humulus scandens</I> was optimized. </LI> <LI> Cellulase amount, inoculation, initial pH value have obvious internal interaction. </LI> <LI> The optimum level of three factors in favor of biohydrogen production was obtained. </LI> </UL> </P>