초록
<P><B>Abstract</B></P> <P>The paper is dedicated to new unconventional sources of non-food renewable feedstock for third generation biofuel – microalgae biomass. Methods of lipid induction in microalgae have been considered. It represents the results of advanced microalgae strains state-of-the-art investigations – lipid producers. The paper discusses selection of stressors at two-stage culturing, where 1st stage includes culturing under most optimal conditions to produce maximum amount of biomass, and at 2nd stage biosynthesis and accumulation of lipids in biomass occurs. Types of stressors used for lipid induction have been briefly reviewed on the basis of foreign studies. Proper experimental results on isolation of new candidate microalgae strains from natural sources, as well as on selection of stressors for lipid induction have been shown and discussed. Nitrogen and phosphoric starvation, high and low illuminance, along with temperature conditions have been studied as stressors. Effectiveness of lipid-containing microalgae screening technique by fluorescent dye Nile red staining has been determined. Processible fast-growing <I>Arhtrospira platensis</I> microalgae/cyanobacteria use perspectiveness as a model for selection of stressors and overall lipid-containing microalgae culturing mode have been verified. Method of various microalgae heat-treated biomass conversion into biohydrogen by anaerobic acetone-butanol fermentation using cellular immobilized catalysts based on <I>Clostridium acetobutylicum</I> has been considered. Ultimate hydrogen efficiency has been found for <I>Chlorococcum</I> sp. <I>rsemsu Ccc-7/11 strain</I> biomass (8.4 и 6.1 mmol/l of medium/day); while hydrogen production is 23.7% and 18.6% of notionally possible level, <I>A. platensis</I> strains cultivated under the influence of stressors (<I>rsemsu 1/02-P</I> – 5.6 mmol/l of medium/day, 32.3%; <I>rsemsu 1/02-Т</I> – 3.3 mmol/of medium/day, 28.8%) rank slightly below them in efficiency, outperforming in hydrogen production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lipid induction in microalgae by means selection of stressors at two-stage culturing have been considered. </LI> <LI> Effectiveness of lipid-containing microalgae screening technique by fluorescent dye Nile red staining has been determined. </LI> <LI> Positive stress reaction have been demonstrated by eight strains of microalgae. </LI> <LI> Some of investigated cultures accumulated the neutral lipids on commercial scale. </LI> <LI> Conversion of biomass residues after isolation of lipids to biohydrogen is effective. </LI> </UL> </P>