초록
<P><B>Abstract</B></P> <P> <I>Trichoderma reesei</I> is a widely used cellulase producer, and development of robust strains for improved cellulase production is of great interest. In this study, the gene <I>Trvib-1</I> encoding a putative transcription factor was overexpressed in <I>T. reesei</I> Rut-C30, and effects on cellulase production by the manipulation as well as corn stover degradation by the crude enzyme were investigated. Cellulase production and protein secretion were significantly improved in the culture of the recombinant <I>T. reesei</I> Vib-1, which were 200% and 219%, respectively, higher than that produced by the parent strain. Cellulase induction was enhanced in the presence of pure cellulose as well as various soluble inducers. Glucose released from the pretreated corn stover hydrolyzed by the crude enzyme in the recombinant strain was improved 40%. These results indicate that the overexpression of <I>Trvib-1</I> is a feasible strategy for producing cellulase to enhance bioconversion efficiency of lignocellulosic biomass.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Overexpression of <I>Trvib-1</I> improved cellulase production in <I>T. reesei</I> Rut-C30. </LI> <LI> Higher cellulase induction was observed using both cellulose and soluble inducers. </LI> <LI> Enhanced sugar release from corn stover was achieved by <I>Trvib-1</I> overexpression. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>