BioChem - DOS

Bioconversion of Beetle-Killed Lodgepole Pine Using SPORL: Process Scale-up Design, Lignin Coproduct, and High Solids Fermentation without Detoxification

Industrial & engineering chemistry research

Zhou, Haifeng; Zhu, J. Y.; Luo, Xiaolin; Leu, Shao-Yuan; Wu, Xiaolei; Gleisner, Roland; Dien, Bruce S.; Hector, Ronald E.; Yang, Dongjie; Qiu, Xueqing; Horn, Eric; Negron, Jose

<P>Mountain pine beetle killed Lodgepole pine (<I>Pinus contorta</I> Douglas ex Loudon) wood chips were pretreated using an acidic sulfite solution of approximately pH = 2.0 at a liquor to wood ratio of 3 and sodium bisulfite loading of 8 wt % on wood. The combined hydrolysis factor (CHF), formulated from reaction kinetics, was used to design a scale-up pretreatment on 2000 g wood chips at a relatively low temperature of 165 &deg;C that reduced furan formation and facilitated high solids saccharification and fermentation. The pretreated solids and liquor were disk milled together to result in a biomass whole slurry of 25% total solids. The whole biomass slurry was directly used to conduct simultaneous enzymatic saccharification and combined fermentation (SSCombF) using a commercial cellulase and <I>Saccharomyces cerevisiae</I> YRH400 without detoxification. A terminal ethanol titer of 47.1 g L<SUP>&ndash;1</SUP> with a yield of 306 L (tonne wood)<SUP>&minus;1</SUP>, or 72.0% theoretical, was achieved when SSCombF was conducted at an unwashed solids loading of 18%. The lignosulfonate (LS) from SPORL was highly sulfonated and showed better dispersibility than a high purity commercial softwood LS, and therefore has potential as a directly marketable coproduct.</P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ie402873y'>ACS Electronic Supporting Info</A></P>

2013

American Chemical Society

0888-5885

1520-5045

52

45

pp.16057-16065

10.1021/ie402873y

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART67412162