BioChem - DOS

Electrofiltration as a purification strategy for microbial poly-(3-hydroxybutyrate)

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Gozke, G.; Prechtl, C.; Kirschhofer, F.; Mothes, G.; Ondruschka, J.; Brenner-Weiss, G.; Obst, U.; Posten, C.

The biodegradable polyester poly-(3-hydroxybutyrate) (PHB), produced by Ralstonia eutropha in batch and fed-batch processes, was purified by electrofiltration. The protein film on PHB granules determines their high negative zeta potential, enabling the application of electrofiltration as an integrated technology in the downstream processing of PHB. In order to determine the optimal purification parameters, various pressure and electric field strength conditions were tested. Electrofiltration of PHB at 4bars and 4V/mm provided an up to four times higher concentration factor than conventional filtration. FT-Raman spectroscopy demonstrated that electrofiltration did not result in structural changes to the products. The study demonstrates the efficiency and practical advantages of electrofiltration as a promising downstream step in the PHB production technology.

2012

Elsevier Applied Science

0960-8524

123

pp.272-278

10.1016/j.biortech.2012.07.039

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART64153472

Electrofiltration; Poly-(3-hydroxybutyrate); Ralstonia eutropha; Purification