초록
<P><B>Abstract</B></P> <P>To expand the diversity of chemical compounds produced through microbial conversion, a platform pathway for the production of widely used industrial chemicals, 1,3-diols, was engineered in <I>Escherichia coli</I>. The pathway was designed by modifying the previously reported (<I>R</I>)-1,3-butanediol synthetic pathway to consist of <I>pct</I> (propionate CoA-transferase) from <I>Megasphaera elsdenii</I>, <I>bktB</I> (thiolase), <I>phaB</I> (NADPH-dependent acetoacetyl-CoA reductase) from <I>Ralstonia eutropha</I>, <I>bld</I> (butyraldehyde dehydrogenase) from <I>Clostridium saccharoperbutylacetonicum</I>, and the endogenous alcohol dehydrogenase(s) of <I>E. coli</I>. The recombinant <I>E. coli</I> strains produced 1,3-pentanediol, 4-methyl-1,3-pentanediol, and 1,2,4-butanetriol, together with 1,3-butanediol, from mixtures of glucose and propionate, isobutyrate, and glycolate, respectively, in shake flask cultures. To the best of our knowledge, this is the first report of microbial production of 1,3-pentanediol and 4-methyl-1,3-pentanediol.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A platform pathway for 1,3-diol production was constructed in <I>Escherichia coli</I>. </LI> <LI> The engineered <I>E. coli</I> produced 4.43mM 1,3-pentanediol. </LI> <LI> The engineered <I>E. coli</I> produced 702μM 4-methyl-1,3-pentanediol. </LI> <LI> The engineered <I>E. coli</I> produced 36.5μM 1,2,4-butanetriol. </LI> </UL> </P>