BioChem - DOS

Lactic acid production from pretreated hydrolysates of corn stover by a newly developed Bacillus coagulans strain

PloS one

Jiang, Ting; Qiao, Hui; Zheng, Zhaojuan; Chu, Qiulu; Li, Xin; Yong, Qiang; Ouyang, Jia

<P>An inhibitor-tolerance strain, <I>Bacillus coagulans</I> GKN316, was developed through atmospheric and room temperature plasma (ARTP) mutation and evolution experiment in condensed dilute-acid hydrolysate (CDH) of corn stover. The fermentabilities of other hydrolysates with <I>B</I>. <I>coagulans</I> GKN316 and the parental strain <I>B</I>. <I>coagulans</I> NL01 were assessed. When using condensed acid-catalyzed steam-exploded hydrolysate (CASEH), condensed acid-catalyzed liquid hot water hydrolysate (CALH) and condensed acid-catalyzed sulfite hydrolysate (CASH) as substrates, the concentration of lactic acid reached 45.39, 16.83, and 18.71 g/L by <I>B</I>. <I>coagulans</I> GKN316, respectively. But for <I>B</I>. <I>coagulans</I> NL01, only CASEH could be directly fermented to produce 15.47 g/L lactic acid. The individual inhibitory effect of furfural, 5-hydroxymethylfurfural (HMF), vanillin, syringaldehyde and <I>p</I>-hydroxybenzaldehyde (pHBal) on xylose utilization by <I>B</I>. <I>coagulans</I> GKN316 was also studied. The strain <I>B</I>. <I>coagulans</I> GKN316 could effectively convert these toxic inhibitors to the less toxic corresponding alcohols <I>in situ</I>. These results suggested that <I>B</I>. <I>coagulans</I> GKN316 was well suited to production of lactic acid from undetoxified lignocellulosic hydrolysates.</P>

2016

Public Library of Science

cc-by

1932-6203

11

2

pp.e0149101

10.1371/journal.pone.0149101

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART87648721

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0149101&type=printable