BioChem - DOS

Improving the system performance of the asymmetric biosynthesis of D-pantoic acid by using artificially self-assembled enzymes in Escherichia coli

ACS biomaterials science & engineering

Li, Tao; Li, Ruifeng; Zhu, Tong; Cui, Xuexian; Li, Chuijian; Cui, Yinglu; Wu, Bian

<P><SMALL>D</SMALL>-Pantoic acid (<SMALL>D</SMALL>-PA) is an important chiral precursor of a broad range of biologically active compounds. The asymmetric synthesis of <SMALL>D</SMALL>-PA through reductase coupling with NADPH regeneration systems is highly promising, but the process is restricted by expensive cofactor consumption and low cofactor recycling frequency. Here, an effective construction of self-assembled ketopantoic acid reductase and glucose dehydrogenase via protein-peptide interaction of PDZ domain and PDZ ligand was established. The self-assembled enzymes exhibited highly ordered two-dimensional threadlike macromolecular structures with improved cofactor regeneration. Furthermore, the bioconversion with whole-cell catalysis showed that the robustness and efficiency of the system with self-assembled enzymes were significantly higher than those of the unassembled enzymes. This study provides a strategy for the effective asymmetric biosynthesis of <SMALL>D</SMALL>-PA with a trace amount of cofactor and shows potential for industrial applications.</P><BR>[FIG OMISSION]</BR>

2020

American Chemical Society

2373-9878

6

1

pp.219-224

10.1021/acsbiomaterials.9b01754

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART98697369

d-pantoic acid; ketopantoic acid reductase; self-assembly; cofactor recycle