BioChem - DOS

Ginsenoside F1 production from ginsenoside Rg1 by a purified β-glucosidase from Fusarium moniliforme var. subglutinans

Biotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology

Kim, Yeong-Su; Yoo, Mi-Hyun; Lee, Gi-Woong; Choi, Jin-Geun; Kim, Kyoung-Rok; Oh, Deok-Kun

<P>Fusarium moniliforme var. subglutinans was selected from among 100 strains of fungi for producing ginsenoside F(1) from ginsenoside Rg(1). The enzyme responsible was purified as a single 85 kDa band with a specific activity of 136 U mg(-1). It hydrolysed glucose-linked ginsenosides Rb(1), Rd and Rg(1) but not for other monosaccharide-linked ginsenosides, Rb(2), Rc, R(1), and Re. Under the optimum conditions of pH 6.0, 50 C, 30 U l(-1) of enzyme, and 5 mg Rg(1) ml(-1), 4 mg F(1) ml(-1) was produced after 4 h, with a molar yield of 100% and a productivity of 1 g l(-1) h(-1). This represents the highest productivity and conversion yield of F(1) yet reported.</P>

2011

Springer-Verlag

0141-5492

1573-6776

33

12

pp.2457-2461

10.1007/s10529-011-0719-0

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