BioChem - DOS

Reactive extraction of amino acids mixture in hydrolysate from cottonseed meal with di(2-ethylhexyl) phosphoric acid

Journal of chemical technology and biotechnology

Zhang, Jian; Zhang, Shunxi; Yang, Xinni; Qiu, Ling; Gao, Bing; Li, Rui; Chen, Jiwang

<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P>In recent years there has been a noticeable growth of amino acid utilization throughout the world. How to separate and purify amino acids from fermentation broths or protein hydrolysates in an effective and low cost way is increasingly becoming an urgent problem. The utilization of solvent extraction technology for amino acids separation offers one solution.</P><P><B>RESULTS</B></P><P>Extractions of individual amino acids in aqueous solution with di(2&#8208;ethyl&#8208;hexyl)phosphoric acid (D2EPHA) are dominated by aqueous pH and their hydrophobicity. The degree of extraction of amino acids increases with increasing pH (1.0<pH<3.0), but changes only slightly in the range 3.0<pH<7.0. Amino acids with a longer carbon chain or cyclic structure have higher degrees of extraction, while those with additional polar groups have lower degrees of extraction. Protein hydrolysate from cottonseed meal was obtained by combination of microbial fermentation and HCl hydrolysis. The greatest extraction of amino acids from the hydrolysate was found at initial feed pH 6.5, and byproducts (e.g. reducing sugars and sodium chloride) were completely retained in raffinate. Amino acids loading the organic phase can be completely stripped by using an aqueous solution having an equal volume of 0.5 mol L<SUP>&minus;1</SUP> HCl.</P><P><B>CONCLUSION</B></P><P>Use of D2EPHA reactive extraction enabled avoidance of a sequence of purification stages and provided purer back&#8208;extracts. &copy; 2014 Society of Chemical Industry</P>

2016

John WileySons, Ltd

0268-2575

1097-4660

91

2

pp.483-489

10.1002/jctb.4602

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74767585

cottonseed meal; hydrolysate; amino acid; di(2&#x2010; ethyl&#x2010; hexyl)phosphoric acid; solvent extraction;