BioChem - DOS

Increase in furfural tolerance by combinatorial overexpression of NAD salvage pathway enzymes in engineered isobutanol-producing E. coli

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Song, Hun-Suk; Jeon, Jong-Min; Kim, Hyun-Joong; Bhatia, Shashi Kant; Sathiyanarayanan, Ganesan; Kim, Junyoung; Won Hong, Ju; Gi Hong, Yoon; Young Choi, Kwon; Kim, Yun-Gon; Kim, Wooseong; Yang, Yung-Hun

<P><B>Abstract</B></P> <P>To reduce the furfural toxicity for biochemical production in <I>E. coli</I>, a new strategy was successfully applied by supplying NAD(P)H through the nicotine amide salvage pathway. To alleviate the toxicity, nicotinamide salvage pathway genes were overexpressed in recombinant, isobutanol-producing <I>E. coli</I>. Gene expression of <I>pncB</I> and <I>nadE</I> respectively showed increased tolerance to furfural among these pathways. The combined expression of <I>pncB</I> and <I>nadE</I> was the most effective in increasing the tolerance of the cells to toxic aldehydes. By comparing <I>noxE</I>- and <I>fdh</I>-harbouring strains, the form of NADH, rather than NAD<SUP>+</SUP>, was the major effector of furfural tolerance. Overall, this study is the application of the salvage pathway to isobutanol production in the presence of furfural, and this system seems to be applicable to alleviate furfural toxicity in the production of other biochemical.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The increased gene expression of <I>pncB</I> and <I>nadE</I> reduced the toxicity of furfural. </LI> <LI> Overexpression of <I>pncB</I> and <I>nadE</I> also reduced inhibitory effect of vanillin, 4-hydroxybenzaldehyde. </LI> <LI> NADH is more important than NAD<SUP>+</SUP> in increasing the tolerance to furfural. </LI> <LI> NAD<SUP>+</SUP> salvage pathway genes is applicable to isobutanol production in the presence of furfural. </LI> </UL> </P>

2017

Elsevier

0960-8524

245

2

pp.1430-1435

10.1016/j.biortech.2017.05.197

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART78806246

Furfural; NADH; pncB; nadE; E. coli; Isobutanol