초록
<P>The coupling of the enantioselective reduction catalyzed by Old Yellow Enzymes (OYEs), together with the in situ substrate feeding product removal (SFPR) concept, significantly improved the productivity of the g-scale preparation of ethyl (<I>S</I>)-2-ethoxy-3-(<I>p</I>-methoxyphenyl)propanoate (EEHP), an important precursor of several PPAR-α/γ agonists, such as Tesaglitazar. The OYEs and the glucose dehydrogenase for cofactor regeneration were cloned, overexpressed in <I>Escherichia coli</I>, and purified. The synthetic sequence was completed by a NaClO<SUB>2</SUB> oxidation employing cheap and environmentally friendly conditions. The product was obtained in 94% yield and with an ee of 98% over the two steps.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/oprdfk/2012/oprdfk.2012.16.issue-2/op200085k/production/images/medium/op-2011-00085k_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/op200085k'>ACS Electronic Supporting Info</A></P>