BioChem - DOS

The role of aldehyde/alcohol dehydrogenase (AdhE) in ethanol production from glycerol by Klebsiella pneumoniae

Journal of industrial microbiology & biotechnology

Oh, Baek-Rock; Hong, Won-Kyung; Heo, Sun-Yeon; Joe, Min-ho; Seo, Jeong-Woo; Kim, Chul Ho

<P><B>Abstract</B><P>Transcriptome analysis of a K. pneumoniae GEM167 mutant strain derived by irradiation with gamma rays, which exhibited high-level production of ethanol from glycerol, showed that the mutant expressed AdhE at a high level. Ethanol production decreased significantly, from 8.8 to 0.5 g l&#x2212;1, when an adhE-deficient derivative of that strain was grown on glycerol. Bacterial growth was also reduced under such conditions, showing that AdhE plays a critical role in maintenance of redox balance by catalyzing ethanol production. Overexpression of AdhE enhanced ethanol production, from pure or crude glycerol, to a maximal level of 31.9 g l&#x2212;1 under fed-batch fermentation conditions; this is the highest level of ethanol production from glycerol reported to date.</P></P>

2013

Oxford University Press

1367-5435

1476-5535

40

2

pp.227-233

10.1007/s10295-012-1224-8

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