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Enrichment of anaerobic syngas-converting bacteria from thermophilic bioreactor sludge

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      2. 연료
    • 화장품용 기능성소재
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논문

Enrichment of anaerobic syngas-converting bacteria from thermophilic bioreactor sludge

학술지

FEMS microbiology ecology

저자명

Alves, Joana I.; Stams, Alfons J.M.; Plugge, Caroline M.; Madalena Alves, M.; Sousa, Diana Z.

초록

<P><B>Abstract</B></P><P>Thermophilic (55?&deg;C) anaerobic microbial communities were enriched with a synthetic syngas mixture (composed of CO, H<SUB>2</SUB>, and CO<SUB>2</SUB>) or with CO alone. Cultures T&#8208;Syn and T&#8208;CO were incubated and successively transferred with syngas (16 transfers) or CO (9 transfers), respectively, with increasing CO partial pressures from 0.09 to 0.88?bar. Culture T&#8208;Syn, after 4 successive transfers with syngas, was also incubated with CO and subsequently transferred (9 transfers) with solely this substrate &ndash; cultures T&#8208;Syn&#8208;CO. Incubation with syngas and CO caused a rapid decrease in the microbial diversity of the anaerobic consortium. T&#8208;Syn and T&#8208;Syn&#8208;CO showed identical microbial composition and were dominated by <I>Desulfotomaculum</I> and <I>Caloribacterium</I> species. Incubation initiated with CO resulted in the enrichment of bacteria from the genera <I>Thermincola</I> and <I>Thermoanaerobacter</I>. Methane was detected in the first two to three transfers of T&#8208;Syn, but production ceased afterward. Acetate was the main product formed by T&#8208;Syn and T&#8208;Syn&#8208;CO. Enriched T&#8208;CO cultures showed a two&#8208;phase conversion, in which H<SUB>2</SUB> was formed first and then converted to acetate. This research provides insight into how thermophilic anaerobic communities develop using syngas/CO as sole energy and carbon source can be steered for specific end products and subsequent microbial synthesis of chemicals.</P>

발행연도

2013

ISSN

0168-6496

ISSN

1574-6941

86

3

페이지

pp.590-597

주제어

synthesis gas; syngas; carbon monoxide; anaerobic degradation; microbial diversity; microbial physiology

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1 2023-12-11
2 2023-12-11

논문; 2013-08-23

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