Pretreatment of sugarcane bagasse with acidified aqueous glycerol solution was evaluated at both laboratory and pilot scales. Laboratory scale pretreatment (4.00g dry mass in 40.00g liquid) with glycerol solutions containing ≤20wt.% water and 1.2wt.% HCl at 130<SUP>o</SUP>C for 60min resulted in biomass having glucan digestibilities of ≥88%. Comparable glucan enzymatic digestibility of 90% was achieved with bagasse pretreated at pilot scale (10kg dry mass in 60kg liquid) using a glycerol solution containing 0.4wt.% HCl and 17wt.% water at 130<SUP>o</SUP>C for 15min. We attribute more efficient pretreatment at pilot scale (despite shorter reaction time and reduced acid content) to improved mixing and heat transfer in a horizontal reactor. Pretreatment of sugarcane bagasse with acid-catalysed glycerol solutions likely produces glycerol-glycosides, which together with hydrolysed lignin are potential substrates for the production of biopolymers.