BioChem - DOS

A three-enzyme system involving an ene-reductase for generating valuable chiral building blocks

Advanced synthesis & catalysis

Mangan, David; Miskelly, Iain; Moody, Thomas S.

<P><B>Abstract</B></P><P>The use of ene&#8208;reductase (ERED) enzymes for the asymmetric reduction of olefins offers a green, renewable alternative to metal&#8208;catalysed asymmetric reduction. We report herein the first example of an ERED&#8208;catalysed enantiospecific reduction carried out at large scale using a carbonyl reductase (CRED) enzyme in the cofactor recycle. This reaction has been paired with a hydrolase&#8208;mediated regioselective ester hydrolysis to generate a valuable chiral building block using a straightforward one&#8208;pot process.</P>

2012

WILEY&#x2010;VCH Verlag

1615-4150

1615-4169

354

11

pp.2185-2190

10.1002/adsc.201101006

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART63895972

asymmetric synthesis; biocatalysis; chirality; enzyme catalysis; hydrogenation; hydrolases; reduction