BioChem - DOS

Novel spectrophotometric method for detection and estimation of butanol in acetone-butanol-ethanol fermenter

Talanta

Maiti, S.; Sarma, S.J.; Brar, S.K.; Bihan, Y.L.; Drogui, P.; Buelna, G.; Verma, M.; Soccol, C.R.

A new, simple, rapid and selective spectrophotometric method has been developed for detection and estimation of butanol in fermentation broth. The red colored compound, produced during reduction of diquat-dibromide-monohydrate with 2-mercaptoethanol in aqueous solution at high pH (>13), becomes purple on phase transfer to butanol and gives distinct absorption at &lambda;<SUB>520nm</SUB>. Estimation of butanol in the fermentation broth has been performed by salting out extraction (SOE) using saturated K<SUB>3</SUB>PO<SUB>4</SUB> solution at high pH (>13) followed by absorbance measurement using diquat reagent. Compatibility and optimization of diquat reagent concentration for detection and estimation of butanol concentration in the fermentation broth range was verified by central composite design. A standard curve was constructed to estimate butanol in acetone-ethanol-butanol (ABE) mixture under optimized conditions. The spectrophotometric results for butanol estimation, was found to have 87.5% concordance with the data from gas chromatographic analysis.

2015

Pergamon Press ; Elsevier Science Ltd

0039-9140

1873-3573

141

pp.116-121

10.1016/j.talanta.2015.03.062

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72051450

Diquat reagent; Salting out extraction (SOE); Spectrophotometric method; Butanol estimation; Real fermenter