초록
<P><B>Abstract</B></P> <P>Amphipathic lignin derivatives (A-LDs) prepared from the black liquor of soda pulping of Japanese cedar are strong accelerators for bioethanol production under a fed-batch simultaneous enzymatic saccharification and fermentation (SSF) process. To improve the bioethanol production concentration, conditions such as reaction temperature, stirring program, and A-LDs loadings were optimized in both small scale and large scale fed-batch SSF. The fed-batch SSF in the presence of 3.0g/L A-LDs at 38°C gave the maximum ethanol production and a high enzyme recovery rate. Furthermore, a jar-fermenter equipped with a powerful mechanical stirrer was designed for 1.5L-scale fed-batch SSF to achieve rigorous mixing during high substrate loading. Finally, the 1.5L fed-batch SSF with a substrate loading of 30% (w/v) produced a high ethanol concentration of 87.9g/L in the presence of A-LDs under optimized conditions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> AL-Ds accelerate the bioethanol production on large scale fed-batch SSF. </LI> <LI> Various parameters for small and large scale fed-batch SSF were highly optimized. </LI> <LI> 30% high substrate loading was achieved by using a custom jar-fermenter. </LI> <LI> Lignocellulosic bioethanol fermentation reached high concentration of 87.9g/L. </LI> </UL> </P>