초록
<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P>Dyed used cotton textiles is a waste material associated with environmental problems. In this study, waste dyed cotton fabrics were used as feedstock for production of bacterial nanocellulose (BNC) with <I>Gluconacetobacter xylinus</I> and production of enzymes with <I>Trichoderma reesei</I> via enzymatic saccharification.</P><P><B>RESULTS</B></P><P>Reactive dyes caused almost no inhibition of the cellulase activity at a concentration of 5 g L<SUP>−1</SUP>, but decreased the BNC production at concentrations higher than 1 g L<SUP>−1</SUP>. The BNC yield reached 12.8 g L<SUP>−1</SUP> with cotton hydrolysate, which was 48% higher than with glucose‐based medium. The spent fermentation broth after BNC harvest was subsequently utilized for enzyme production. Cellulase activities produced by <I>T. reesei</I> reached 5.3 U mL<SUP>−1</SUP> with spent detoxified purple bed sheet (PBS) hydrolysate, and 8.2 U mL<SUP>−1</SUP> with 2‐fold diluted spent PBS hydrolysate, which was almost the same or higher than with glucose medium (5.6 U mL<SUP>−1</SUP>). The xylanase activities (60.2 U mL<SUP>−1</SUP> and 88.0 U mL<SUP>−1</SUP>) obtained with the two media were 3–4 times higher than that obtained with glucose medium (21.0 U mL<SUP>−1</SUP>).</P><P><B>CONCLUSION</B></P><P>This approach could contribute to economical conversion of cellulosic waste to two high value‐added microbial products, while also providing new raw materials for a more sustainable textile industry. © 2015 Society of Chemical Industry</P>