Development of a hybrid fermentation-enzymatic bioprocess for the production of ethyl lactate from dairy waste

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바이오화학분류

- 바이오플라스틱
  1. 플라스틱
- 바이오정밀화학
  1. 용매
  2. 화학제품
  3. 연료
  4. 기타
- 화장품용 기능성소재
  1. 계면활성제⁄증점제
- 의료용 화학소재
  1. 치료제
  2. 식품첨가제

논문

Development of a hybrid fermentation-enzymatic bioprocess for the production of ethyl lactate from dairy waste

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Koutinas, M.; Menelaou, M.; Nicolaou, E.N.

초록

This work explores the potential for the development of a hybrid fermentation-enzymatic process for the production of ethyl lactate from dairy waste. Cheese whey was used in Kluyveromyces marxianus and Lactobacillus bulgaricus batch cultures to produce ethanol and lactic acid respectively. Subsequently, the fermentation products were transferred into an organic phase through liquid-liquid extraction and ethyl lactate was formed in an esterification reaction catalyzed by lipases. The production of ethanol and lactic acid achieved under different conditions was 23gL-1 and 29gL-1, respectively. Furthermore, the efficiency of various organic solvents for the esterification reaction was evaluated and toluene was chosen for application in the process. The effect of water content was determined aiming to maximize the product yield and 40mgml-1 was the optimal enzyme concentration. The bioprocess achieved maximum conversion of 33% constituting a valuable alternative to the application of energy demanding chemically derived methods.

발행연도

2014

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

165

페이지

pp.343-349

DOI

10.1016/j.biortech.2014.03.053