Metabolic Engineering toward Sustainable Production of Nylon-6
메타 데이터
바이오화학분류
바이오플라스틱
고무
플라스틱
바이오정밀화학
화학제품
의료용 화학소재
치료제
식품첨가제
논문
Metabolic Engineering toward Sustainable Production of Nylon-6
학술지
ACS Synthetic biology
저자명
Turk, Stefan C. H. J.; Kloosterman, Wigard P.; Ninaber, Dennis K.; Kolen, Karin P. A. M.; Knutova, Julia; Suir, Erwin; Schü rmann, Martin; Raemakers-Franken, Petronella C.; Mü ller, Monika; de Wildeman, Stefaan M. A.; Raamsdonk, Leonie M.; van der Pol, Ruud; Wu, Liang; Temudo, Margarida F.; van der Hoeven, Rob A. M.; Akeroyd, Michiel; van der Stoel, Roland E.; Noorman, Henk J.; Bovenberg, Roel A. L.; Trefzer, Axel C.
초록
<P>Nylon-6 is a bulk polymer used for many applications. It consists of the non-natural building block 6-aminocaproic acid, the linear form of caprolactam. Via a retro-synthetic approach, two synthetic pathways were identified for the fermentative production of 6-aminocaproic acid. Both pathways require yet unreported novel biocatalytic steps. We demonstrated proof of these bioconversions by <I>in vitro</I> enzyme assays with a set of selected candidate proteins expressed in <I>Escherichia coli</I>. One of the biosynthetic pathways starts with 2-oxoglutarate and contains bioconversions of the ketoacid elongation pathway known from methanogenic archaea. This pathway was selected for implementation in <I>E. coli</I> and yielded 6-aminocaproic acid at levels up to 160 mg/L in lab-scale batch fermentations. The total amount of 6-aminocaproic acid and related intermediates generated by this pathway exceeded 2 g/L in lab-scale fed-batch fermentations, indicating its potential for further optimization toward large-scale sustainable production of nylon-6.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/asbcd6/2016/asbcd6.2016.5.issue-1/acssynbio.5b00129/production/images/medium/sb-2015-00129a_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/sb5b00129'>ACS Electronic Supporting Info</A></P>