Engineering Escherichia coli cells for cellobiose assimilation through a phosphorolytic mechanism
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Engineering Escherichia coli cells for cellobiose assimilation through a phosphorolytic mechanism
학술지
Applied and environmental microbiology
저자명
Sekar, Ramanan; Shin, Hyun-Dong; Chen, Rachel
초록
<P><B>ABSTRACT</B><P> We report here the first engineering effort for Escherichia coli biocatalysts to assimilate cellobiose through a phosphorolytic mechanism. Cytoplasmic expression of the Saccharophagus cellobiose phosphorylase was shown to enable E. coli to use cellobiose. Subsequent knockout and complementation studies provided solid evidence that the endogenous LacY was responsible for the transport of cellobiose. </P></P>