BioChem - DOS

High versus low level expression of the lycopene biosynthesis genes from Pantoea ananatis in Escherichia coli

Biotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology

Albermann, Christoph

<P>The heterologous synthesis of lycopene in non-carotenogenic Escherichia coli required the introduction of the biosynthesis genes crtE, crtB, and crtI. Recombinant E. coli strains, expressing each lycopene biosynthesis gene from Pantoea ananatis using multi-copy plasmid or single-copies after stable chromosomal integration, were cultivated and the formation of lycopene was investigated. The different expression conditions significantly influenced the lycopene formation as well as the growth behaviour. High plasmid expression levels of crtI with a single copy background of crtE and crtB in E. coli led to a predominate synthesis of tetradehydrolycopene at 253 microg g(-1) (cdw).</P>

2011

Springer-Verlag

0141-5492

1573-6776

33

2

pp.313-319

10.1007/s10529-010-0422-6

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