BioChem - DOS

Amylase production by Aspergillus oryzae in a solid-state bioreactor with fed-batch operation using mussel processing wastewaters as feeding medium

Journal of chemical technology and biotechnology

Torrado, Ana; Vá zquez, José ‐ Antonio; Prieto, Miguel‐ Á ngel; Fuciñ os, Pablo; Montemayor, Marí a‐ Ignacia; Pastrana, Lorenzo; Gonzá lez, Marí a‐ Pilar; Murado, Miguel‐ Á ngel

<P><B>Abstract</B></P><P><B>BACKGROUND: The high oxygen availability in solid&#8208;state cultures makes them especially suitable for fungal enzyme production. Glycogen&#8208;rich mussel processing wastewaters have been used successfully as substrates for amylase production by <I>Aspegillus oryzae</I> in solid&#8208;state cultures supported in polyurethane foam. The aim of this work was to study the fed&#8208;batch mode in a scalable solid&#8208;state bioreactor to extend the productive period and obtain high amylase production. Culture salinization due to the NaCl content of these wastewaters is the main drawback.</B></P><P><B>RESULTS: Evaporation of the excess liquid added during feeding led to progressive salt accumulation. The effect of culture salinization on amylase production was analysed and mathematically modelled, and the <I>IC</I><SUB>50</SUB> (65.4 g L<SUP>&minus;1</SUP> of NaCl) was calculated. An optimum operation mode for this bioreactor was designed that included foam extrusion for removing the incubated medium, washing and a final recharge with fresh medium every 72 h of incubation. This procedure kept the salt concentration under <I>IC</I><SUB>50</SUB> and increased the amylase production from 3000 to 12 000 UE g<SUP>&minus;1</SUP>.</B></P><P><B>CONCLUSION: An operating mode with intermittent extrusions and washings of the support between feedings was found to be an appropriate procedure for preventing the accumulation of inhibitory compounds in fed&#8208;batch solid&#8208;state cultures. &copy; 2012 Society of Chemical Industry</B></P>

2013

John Wiley Sons, Ltd

0268-2575

1097-4660

88

2

pp.226-236

10.1002/jctb.3813

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65414524

amylase production; mussel processing wastewaters; solid&#x2010; state; salinization; solid&#x2010; state bioreactor; fed&#x2010; batch; Weibull equation; SSC; SSF;