초록
<P><B>Abstract</B></P> <P>Several problems are being encountered in the submerged fermentation (SMF) for cellulase production, globally. In this work, in a laboratory scale operation, three different fed-batch strategies were demonstrated to attempt circumventing the problems related to a batch SMF. The processes used novel surgical waste cotton and cardboard substrate mixture and a cellulolytic fungus <I>Trichoderma harzanium ATCC®</I> 20846™. FPase activities of: 1.94 ± 0.05 IU/mL, 2.12 ± 0.07 IU/mL, and 1.92 ± 0.2 IU/mL were obtained for the exponential feeding strategy; pulse feeding strategy, and pH stat, respectively. For all the three fed-batch SMFs, the biomass yield coefficients (Y<SUB>x/s</SUB>, Y<SUB>x/O2</SUB>); Oxygen Uptake Rates (OUR); maintenance coefficients (mO2, ms); mass transfer co-efficients (KLa); Oxygen Transfer Rates (OTR); and the effects of viscosity, sugar accumulation, and agitation on cellulase production; were estimated. Pulse feeding strategy yielded enhancements in enzyme activities than the other two feeding strategies. Basic financial analyses have been presented.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Comparison of fed batch processes, based on a model; indirect feed back loop; and microbial substrate uptake rate. </LI> <LI> Demonstration of fed batch bioprocesses for an enhanced cellulase yield from a waste biomass mixture. </LI> <LI> Presentation of the significant kinetic values of substrate utilization and fermentation. </LI> <LI> Presentation of basic financial analyses of the bioprocesses. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>