BioChem - DOS

Partition of proteases from Lentinus citrinus DPUA 1535 by the PEG/phosphate aqueous two-phase system

Química Nova

Kirsch Larissa de Souza; Pinto Ana Carolina dos Santos; Teixeira Maria Francisca Simas; Porto Tatiana Souza; Porto Ana Lúcia Figueiredo

A full two-level factorial design was employed to study the influence of PEG molar mass (MM PEG), PEG concentration (C PEG) and phosphate concentration (C PHOSPH) on proteases partition by Lentinus citrinus DPUA 1535 in a PEG/phosphate aqueous two-phase system (ATPS). For all ATPS studied, proteases partitioned for the top phase and the best proteases extraction condition was obtained with MM PEG = 6000 g mol-1, C PEG = 17.5% (w/w) and C PHOSPH = 25% (w/w) with (1.1) purification factor and (151%) activity yield. Findings reported here demonstrate a practical strategy that serves as a first step for proteases purification from crude extract by L. citrinus.

2012

Sociedade Brasileira de Química

cc-by-nc

35

10

pp.1912-1915

10.1590/s0100-40422012001000004

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=ATN0014806739

https://www.scielo.br/j/qn/a/xLkk8pJxy7XVhbZppMxvqXK/?lang=en&format=pdf