초록
<P><B>Abstract</B></P> <P>Green liquor (GL) combined with H<SUB>2</SUB>O<SUB>2</SUB> (GL-H<SUB>2</SUB>O<SUB>2</SUB>) and green liquor (GL) combined with ethanol (GL-ethanol) were chosen for treating sugarcane bagasse. Results showed that the glucose yield (calculated from the glucose content as a percentage of the theoretical glucose available in the substrates)of sugarcane bagasse from GL-ethanol pretreatment (97.7%) was higher than that from GL-H<SUB>2</SUB>O<SUB>2</SUB> pretreatment (41.7%) after 72h hydrolysis with 18 filter paper unit (FPU)/g-cellulose for cellulase, 27,175 cellobiase units (CBU)/g-cellulose for β-glucosidase. Furthermore, about 94.1% of xylan was converted to xylose after GL-ethanol pretreatment without additional xylanase, while the xylose yield was only 29.2% after GL-H<SUB>2</SUB>O<SUB>2</SUB> pretreatment. Scanning electron microscopy showed that GL-ethanol pretreatment could break up the fiber severely. Moreover, GL-ethanol pretreated substrate was more accessible to cellulase and more hydrophilic than that of GL-H<SUB>2</SUB>O<SUB>2</SUB> pretreated. Therefore, GL-ethanol pretreatment is a promising method for improving the overall sugar (glucose and xylan) yield of sugarcane bagasse.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Glucose yield was greatly improved after GL-ethanol pretreatment. </LI> <LI> GL-ethanol pretreatment increased xylose yield without additional xylanase. </LI> <LI> The hydrophily of bagasse was significantly increased by GL-ethanol pretreatment. </LI> <LI> GL-ethanol pretreatment increased cellulose accessibility efficiently. </LI> </UL> </P>